Zheng, Gang

Medicine

Evaluation of Clononal Immunoglobulin DNA rearrangements in Circluating Cell-Free DNA in HIV infected Patients with and without Lymphoma

Background: With the evolution of the HIV epidemic in South Africa, rates of HIV-associated lymphoma have dramatically increased. An already overburdened healthcare system is responsible for rising to this challenge. Recent advances in our understanding of circulating cell-free (CCF) DNA hold promise in streamlining the diagnostic approach for AIDS lymphoma.

Methods: In this pilot study we plan to evaluate 20 prospectively collected, South African AIDS lymphoma biopsy specimens and plasma samples for the presence of Immunoglobulin (Ig) DNA clonal rearrangements. As a negative control we plan to evaluate plasma samples from 20 South African HIV patients without lymphoma for the presence of Immunoglobulin (Ig) DNA clonal rearrangements.

Significance: Ig DNA clonality in CCF DNA might serve to help prioritize patients for imaging and biopsy to firmly establish a lymphoma diagnosis and to monitor response to treatment. The findings of this pilot investigation will assess the appropriateness of the techniques in the South African setting and lay the groundwork for a larger prospective study. 

People

Joanne Katz, ScD MS,BSc

Associate Chair, Director of Academic Programs

Robert Bollinger Jr., MD MPH

Director, Johns Hopkins Center for Clinical Global Health Education (CCGHE); Associate Director, Johns Hopkins Center for Global...

Yukari C. Manabe, MD

Associate Director of Global Health Research and Innovation

Stefan Baral, MD MPH,MBA,MSc

Director, Key Populations Program

Caitlin Kennedy, PhD MPH,BA

Co-Director, MPH concentration in Social and Behavioral Sciences in Public Health; Associate Director, Center for Qualitative...

Noreen Hynes, MD MPH

Director, Geographic Medicine Center of the Division of Infectious Diseases
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December 2017

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