Evaluation of Clononal Immunoglobulin DNA rearrangements in Circluating Cell-Free DNA in HIV infected Patients with and without Lymphoma
Background: With the evolution of the HIV epidemic in South Africa, rates of HIV-associated lymphoma have dramatically increased. An already overburdened healthcare system is responsible for rising to this challenge. Recent advances in our understanding of circulating cell-free (CCF) DNA hold promise in streamlining the diagnostic approach for AIDS lymphoma.
Methods: In this pilot study we plan to evaluate 20 prospectively collected, South African AIDS lymphoma biopsy specimens and plasma samples for the presence of Immunoglobulin (Ig) DNA clonal rearrangements. As a negative control we plan to evaluate plasma samples from 20 South African HIV patients without lymphoma for the presence of Immunoglobulin (Ig) DNA clonal rearrangements.
Significance: Ig DNA clonality in CCF DNA might serve to help prioritize patients for imaging and biopsy to firmly establish a lymphoma diagnosis and to monitor response to treatment. The findings of this pilot investigation will assess the appropriateness of the techniques in the South African setting and lay the groundwork for a larger prospective study.